Welcome to The Takeiteasy


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Takeshi Fukuhara YOUNG RESEARCH SCIENTIST
AT THE BENCH

Developmental Biology
by Molecular and Biochemical Approach.

Driving Force

*Moon..............+ fish (=Manbou)

Best at Low Tide, yeah!
  
Academic History

HIGH SCHOOL
Chiba Highschool in Chiba Pref. (JAPAN)


UNDER GRADUATE
Tokyo Institute of Technology,
Faculty of Biosciences and Biotechnology.


GRADUATE
Tokyo Institute of Technology,
Graduate School of Bioscience and Biotechnology


NOW in D3 degree as putative Ph.D.
c/o Dr.Takeo Kishimoto.

Scholarship and FellowShip
01.Takenaka scholarship(1993.4-1997.3)
02.Ito-foundation schorship(1997.4-1999.3)
03.JSPS fellow DC1(1999.4-2002.3)

Awards
T.I.T 120years memorial writing contest(2001)
Publication
#01.
Accepted. Nature Cell Biology, @Oct.19th,2001.

NOW in Press.
publish in 2001 Jan.

Title and Links in detail coming soon.
Skills:
 Techniques below are almost able to Transfer and Establish into any Lab.




Molecular Biology

*DNA work:
**Cloning, 3'and 5'RACE, Sequence,BAC screening, Mutagenise, Sourthern, PCR, degenerative PCR, Genomic Library Construction and Screening,

*mRNA work:
**In Vitro Transcription and Tranlation, Northern,cDNA synthesis,RT-PCR, cDNA Library Construction and Screening,



Cell Biology
to be summarised soon.


Biochemistry

*General methods
Western blotting, Generation of polyclonal antibody(rabbit)and monoclonal antibody(mouse)
*Chromatography
(Manual, Open Column and APB Smart System operating):
**Reversed Phase-HPLC, Gel Filtration, Ion Exchange, Affinity Purification(GST,His6,MBP,Myc etc.)
***Metabolic Labeling using Phosphorus 32 and 35S Met.



*Mass Spectrometry
(Perseptive Voyger DE-RP operating):
**Peptide Analysis and for Determination of phosphorylation site.




Cell Culture and Karyotype analysis

*Cell line:derived from
**Insect, Fish, Salamander, Turtle, Bird, Rat Kangaroo, Mouse, Human




Others

*Fmoc-Peptide Synthesis
**for normal a.a seq, and Phosphorylated seq.
(special thanks to Mr.Obataya and Dr.Mihara technically instructed!)

*Micro Injection
**using starfish oocyte. (Dr.Kishimoto instructing)

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